Music engagement for the purposes of cognitive and emotional regulation has been shown to be consistently associated with, and predictive of, subjective indices of well-being and adaptive emotion regulation strategy.  The present study investigated the extent to which music engagement contributed to neurophysiological substrates of well-being, after statistically controlling first, for age and gender, and secondly, music listening and training in a sample of 38 participants between the ages of 18 and 42 (M = 27.11, SD = 6.66).  Frontal asymmetry and heart rate variability were acquired using electroencephalography (EEG) and electrocardiography (ECG).  Intentional use of music for cognitive and emotional regulation predicted both well-being and emotion regulation capacity, as measured respectively by resting frontal asymmetry and high frequency heart rate variability.  Furthermore, this predictive value was maintained after statistically controlling for age, gender, music listening and music training.  These findings suggest that purposeful engagement with music for cognitive and emotional regulation could be strategically targeted for future well-being interventions.